Journal: Current Issues in Molecular Biology
Article Title: Beeswax Alcohol Prevents Low-Density Lipoprotein Oxidation and Demonstrates Antioxidant Activities in Zebrafish Embryos and Human Subjects: A Clinical Study
doi: 10.3390/cimb46010026
Figure Lengend Snippet: Effect of beeswax alcohol (BWA) and coenzyme Q 10 (CoQ 10 ) on CuSO 4 -induced oxidation of LDL. ( A ) Electrophoretic mobility of apo-B fraction of LDL. Lanes 1, 2, and 3 represent LDL + CuSO 4 treated with 10, 20, and 30 μM CoQ 10 , respectively. Lanes N and O reflect a native non-oxidized LDL and CuSO 4 -oxidized LDL, respectively. Lanes 4, 5, and 6 symbolize LDL + CuSO 4 treated with 10, 20, and 30 μM BWA. Electrophoresis was performed on 0.5% agarose gel using Tris-EDTA buffer (pH 8.0) at a constant voltage (50 V). The red arrow indicates the gel loading front. ( B ) Quantification of CuSO 4 -induced LDL oxidation in the presence and absence of BWA and CoQ 10 . The LDL oxidation was quantified by thiobarbituric acid reactive substance (TBARS) assay using malondialdehyde (MDA) as reference. p value represents the statistical difference between the groups with respect to oxidized LDL employing one-way ANOVA following Tukey’s post hoc analysis.
Article Snippet: 2,2-Diphenyl-1-picrylhydrazyl (Cat. No. 044150), paraoxon-ethyl (Cat. No. 36186), N -ε-carboxymethyllysine (Cat No. 14580-5 g), coenzyme Q 10 (CoQ 10 ) (Cat. No. C9538-100 mg), dihydroethidium (Cat. No. 37291), and acridine orange (Cat. No. A9231) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Techniques: Electrophoresis, Agarose Gel Electrophoresis, TBARS Assay